Part:BBa_K4202028:Experience

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Characterization

Vector construct

To characterization this part, we constructed the measurement device BBa_K4202036 and then constructed it to the pHY300PLK plasmid. The vector was then transformed into Bacillus subtilis WB600 strain.

Characterization method

The transformant were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 0%, 0.1%, 0.5%, 1%, 2%, 5% sucrose (w/v) at the ratio of 1:100. After cultured in 37℃, 220rpm for 7 hours, the culture were diluted into PBS at the ratio of 1:1000 and were measured the fluorescence intensity through flow cytometry. The cluster of Bacillus subtilis should be easily gated from SSC-A vs. FSH-H figure.

Fig 1 The Bacillus subtilis in the SSC-A vs. FSH-H figure

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